A dual gene‐silencing vector system for monocot and dicot plants
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چکیده
منابع مشابه
Dicot and monocot plants differ in retinoblastoma-related protein subfamilies.
The present study supports the view that the retinoblastoma functions are shared by two distinct retinoblastoma-related (RBR) protein subfamilies in the monocot cereal species, whereas dicot plants have only a single RBR protein. Genes encoding RBR proteins were identified and characterized in alfalfa (Medicago sativa), rice (Oryza sativa), and wheat (Triticum aestivum). The alfalfa MsRBR gene ...
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CRISPR/Cas9 genome targeting systems have been applied to a variety of species. However, most CRISPR/Cas9 systems reported for plants can only modify one or a few target sites. Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants. We designed PCR-based procedures t...
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At least two components that modulate plant resistance against the fungal powdery mildew disease are ancient and have been conserved since the time of the monocot-dicot split (≈ 200 Mya). These components are the seven transmembrane domain containing MLO/MLO2 protein and the syntaxin ROR2/PEN1, which act antagonistically and have been identified in the monocot barley (Hordeum vulgare) and the d...
متن کاملImpact of cis-acting elements’ frequency in transcription activity in dicot and monocot plants
The production of new cultivars via recombinant DNA technology is important in applied agriculture. Promoters play fundamental roles in successful transformation and gene expression. Fragments of the upstream regulatory region of the movement protein gene of the Tomato yellow leaf curl virus (TYLCV; two fragments) and Watermelon chlorotic stunt virus (WmCSV, two fragments) and one fragment of t...
متن کاملMolecular analysis of dicot and monocot small nuclear RNA populations.
Oligonucleotides directed against conserved small nuclear RNA (snRNA) sequences have been used to identify the individual U1, U2, U4, U5, and U6 snRNAs in dicot and monocot nuclei. The plant snRNA populations are significantly more heterogeneous than the mammalian or Saccharomyces cerevisiae snRNA populations. U6 snRNA exists as a single species of similar size in monocot and dicot nuclei. The ...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2013
ISSN: 1467-7644,1467-7652
DOI: 10.1111/pbi.12140